Laminin beta 3 Antibody (CL3353)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46622

Novus Biologicals, part of Bio-Techne
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NBP2-46622
NBP2-46622-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Rabbit

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3353

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Laminin beta 3 Antibodies »

Product Specifications

Immunogen

Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence KLVTSMTKQLGDFWTRMEELRHQARQQGAEAVQAQQLAEGASEQALSAQEGFERIKQKYAELKDRLGQSS mlGEQGARIQSVKTEAEELFGETMEMMDRMKDMELELLRGSQAI mlRSADLTGLEKRVEQ

Epitope

ASEQALSAQEGFERI

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin beta 3 Antibody (CL3353)

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP2-46622]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP2-46622]

Immunohistochemistry-Paraffin: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Analysis in human skin and pancreas tissues using NBP2-46622 antibody. Corresponding LAMB3 RNA-seq data are presented for the same tissues.
Western Blot: Laminin beta 3 Antibody (CL3353) [NBP2-46622]

Western Blot: Laminin beta 3 Antibody (CL3353) [NBP2-46622]

Western Blot: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.
Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (CL3353) [NBP2-46622]

Immunocytochemistry/ Immunofluorescence: Laminin beta 3 Antibody (CL3353) [NBP2-46622]

Immunocytochemistry/Immunofluorescence: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Staining of A-431 cells using the Anti-LAMB3 monoclonal antibody, showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
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Applications for Laminin beta 3 Antibody (CL3353)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-46622 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin beta 3

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.

Alternate Names

BM600-125KDA, Epiligrin subunit bata, FLJ99565, Kalinin B1 chain, Kalinin subunit beta, kalinin-140kDa, LAM5, Laminin 5, Laminin B1k chain, laminin S B3 chain, laminin subunit beta-3, laminin, beta 3, laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)), Laminin-5 subunit beta, LAMNB1, Nicein subunit beta, nicein-125kDa

Entrez Gene IDs

3914 (Human)

Gene Symbol

LAMB3

UniProt

Q13751 (Human)

Additional Laminin beta 3 Products

Product Documents for Laminin beta 3 Antibody (CL3353)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Laminin beta 3 Antibody (CL3353)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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