Laminin gamma 1 Antibody (CL3195)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42394

Novus Biologicals, part of Bio-Techne
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NBP2-42394
NBP2-42394-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL3195

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Laminin gamma 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ECREGFVGNRCDQCEENYFYNRSWPGCQECPACYRLVKDKVADHRVKLQELESLIANLGTGDEMVTDQAFEDRLKEAEREVMDLLREAQDVKDVDQNLMDRLQRVNNTLSSQISRLQNIRNTIEETGNLAEQARAHVENTERLIEIASR

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Laminin gamma 1 Antibody (CL3195)

Western Blot: Laminin gamma 1 Antibody (CL3195) [NBP2-42394]

Western Blot: Laminin gamma 1 Antibody (CL3195) [NBP2-42394]

Western Blot: Laminin gamma 1 Antibody (CL3195) [NBP2-42394] - Lane 1: Marker [kDa] 460, 268, 238, 171, 117, 71, 55, 41, 31 Lane 2: Purified human recombinant protein Laminin-111 Lane 3: Purified human recombinant protein Laminin-121 Lane 4: Purified human recombinant protein Laminin-211 Lane 5: Purified human recombinant protein Laminin-221 Lane 6: Purified human recombinant protein Laminin-411 Lane 7: Purified human recombinant protein Laminin-421 Lane 8: Purified human recombinant protein Laminin-511 Lane 9: Purified human recombinant protein Laminin-521 Lane 10: Purified human recombinant protein Laminin-332
Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394] - Staining of human lymph node shows absence of immunoreactivity in lymphoid cells (negative control).
Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3195) [NBP2-42394] - Staining of human rectum shows strong immunoreactivity in basement membrane of glandular epithelium.
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Applications for Laminin gamma 1 Antibody (CL3195)

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin gamma 1

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Alternate Names

LAMC1, Laminin B2

Gene Symbol

LAMC1

UniProt

P11047 (Human)

Additional Laminin gamma 1 Products

Product Documents for Laminin gamma 1 Antibody (CL3195)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Laminin gamma 1 Antibody (CL3195)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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