Laminin S/Laminin beta 2 Antibody (OTI3B4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45583

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3B4

Concentration

1 mg/ml

View all Laminin S Antibodies »

Product Summary for Laminin S/Laminin beta 2 Antibody (OTI3B4)

Immunogen

Human recombinant protein fragment corresponding to amino acids 314-561 of human LAMB2(NP_002283) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

192.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Laminin S/Laminin beta 2 Antibody (OTI3B4)

Western Blot: Laminin S/Laminin beta 2 Antibody (OTI3B4) [NBP2-45583]

Western Blot: Laminin S/Laminin beta 2 Antibody (OTI3B4) [NBP2-45583]

Western Blot: Laminin S/Laminin beta 2 Antibody (3B4) [NBP2-45583] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Laminin S.
Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) [NBP2-45583]

Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) [NBP2-45583]

Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (3B4) [NBP2-45583] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) [NBP2-45583]

Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (OTI3B4) [NBP2-45583]

Immunohistochemistry: Laminin S/Laminin beta 2 Antibody (3B4) [NBP2-45583] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for Laminin S/Laminin beta 2 Antibody (OTI3B4)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Laminin S

The Laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated alpha, beta and gamma. A major component of the basal lamina, Laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues. During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells. The beta-2 laminin chain plays a role in melanoma spread, promoting tumor migration along the abluminal surface of vessel, a phenomenon which has been termed extra-vascular migratory metastasis.

Long Name

Laminin, beta 2

Alternate Names

LAMB2, Laminin beta 2

Gene Symbol

LAMB2

Additional Laminin S Products

Product Documents for Laminin S/Laminin beta 2 Antibody (OTI3B4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Laminin S/Laminin beta 2 Antibody (OTI3B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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