Laminin S/Laminin beta 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87717

Novus Biologicals, part of Bio-Techne
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NBP1-87717
NBP1-87717-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Laminin S Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DLTDVQDENFNANHALSGLERDRLALNLTLRQLDQHLDLLKHSNFLGAYDSIRHAHSQSAEAERRANTSALAVPSPVSNSASARHRTEALMDAQKEDFNSKHMANQRALGKLSAHTHTLSLTDINELVCGAPG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Laminin S/Laminin beta 2 Antibody

Western Blot: Laminin S/Laminin beta 2 Antibody [NBP1-87717]

Western Blot: Laminin S/Laminin beta 2 Antibody [NBP1-87717]

Western Blot: Laminin S/Laminin beta 2 Antibody [NBP1-87717] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP1-87717]

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP1-87717]

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP1-87717] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP1-87717]

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP1-87717]

Immunohistochemistry-Paraffin: Laminin S/Laminin beta 2 Antibody [NBP1-87717] - Staining of human heart muscle shows weak to moderate membranous positivity in cardiomyocytes.
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Applications for Laminin S/Laminin beta 2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin S

The Laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated alpha, beta and gamma. A major component of the basal lamina, Laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues. During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells. The beta-2 laminin chain plays a role in melanoma spread, promoting tumor migration along the abluminal surface of vessel, a phenomenon which has been termed extra-vascular migratory metastasis.

Long Name

Laminin, beta 2

Alternate Names

LAMB2, Laminin beta 2

Gene Symbol

LAMB2

Additional Laminin S Products

Product Documents for Laminin S/Laminin beta 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Laminin S/Laminin beta 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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