LAP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82847

Novus Biologicals, part of Bio-Techne
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NBP1-82847
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LAP3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AGRVVVRRLAVRRFGSRSLSTADMTKGLVLGIYSKEKEDDVPQFTSAGENFDKLLAGKLRETLNISGPPLKAGKTRTFYGLHQDFPSVVLVG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LAP3 Antibody

Western Blot: LAP3 Antibody [NBP1-82847]

Western Blot: LAP3 Antibody [NBP1-82847]

Western Blot: LAP3 Antibody [NBP1-82847] - Analysis using Anti-LAP3 antibody NBP1-82847 (A) shows similar pattern to independent antibody NBP1-82848 (B).
Immunocytochemistry/ Immunofluorescence: LAP3 Antibody [NBP1-82847]

Immunocytochemistry/ Immunofluorescence: LAP3 Antibody [NBP1-82847]

Immunocytochemistry/Immunofluorescence: LAP3 Antibody [NBP1-82847] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & midbody.
Immunohistochemistry-Paraffin: LAP3 Antibody [NBP1-82847]

Immunohistochemistry-Paraffin: LAP3 Antibody [NBP1-82847]

Immunohistochemistry-Paraffin: LAP3 Antibody [NBP1-82847] - Staining of human kidney.
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Applications for LAP3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAP3

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides

Long Name

Cytosol aminopeptidase

Alternate Names

EC 3.4.11.1, EC 3.4.11.5, EC 3.4.13.23, LAPEP, PEPS, Peptidase S

Gene Symbol

LAP3

Additional LAP3 Products

Product Documents for LAP3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LAP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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