LAP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97066

Novus Biologicals, part of Bio-Techne
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NBP2-97066-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LAP3 Antibodies »

Product Summary for LAP3 Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human LAP3 (Accession#: NP_056991.2; Met1-Ala519)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for LAP3 Antibody

Western Blot: LAP3 Antibody [NBP2-97066]

Western Blot: LAP3 Antibody [NBP2-97066]

Western Blot: LAP3 Antibody [NBP2-97066] - Anti-LAP3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 55 kDa
Immunocytochemistry/ Immunofluorescence: LAP3 Antibody [NBP2-97066]

Immunocytochemistry/ Immunofluorescence: LAP3 Antibody [NBP2-97066]

Immunocytochemistry/Immunofluorescence: LAP3 Antibody [NBP2-97066] - Immunofluorescence staining of LAP3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human LAP3 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.
Immunohistochemistry-Paraffin: LAP3 Antibody [NBP2-97066]

Immunohistochemistry-Paraffin: LAP3 Antibody [NBP2-97066]

Immunohistochemistry-Paraffin: LAP3 Antibody [NBP2-97066] - Immunochemical staining of human LAP3 in human liver with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
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Applications for LAP3 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:10000-1:20000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAP3

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides

Long Name

Cytosol aminopeptidase

Alternate Names

EC 3.4.11.1, EC 3.4.11.5, EC 3.4.13.23, LAPEP, PEPS, Peptidase S

Gene Symbol

LAP3

Additional LAP3 Products

Product Documents for LAP3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LAP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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