LARP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19128
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human La ribonucleoprotein domain family, member 1 using the numbering given in entry NP_291029.2 (GeneID 23367).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LARP1 Antibody
Western Blot: LARP1 Antibody [NBP1-19128]
Western Blot: LARP1 Antibody [NBP1-19128] - The identification of host proteins immunoprecipitated with L1 ORF1p-FLAG. Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Primary antibodies used to probe western blots are indicated to the left of the images (ahnRNPL (NBP1-67852), aILF3 (EPR3627), aDHX9 (NB110-40579), aMATR3 (NB100-1761), aNCL (NB100-1920)). Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1005121), licensed under a CC-BY license.Immunoprecipitation: LARP1 Antibody [NBP1-19128]
Immunoprecipitation: LARP1 Antibody [NBP1-19128] - Detection of human LARP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LARP1 NBP1-19128 used for IP at 3 ug per reaction. LARP1 was also immunoprecipitated by rabbit anti-LARP1 antibody NBP1-19129. For blotting immunoprecipitated LARP1, NBP1-19128 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Immunohistochemistry-Paraffin: LARP1 Antibody [NBP1-19128] -
Immunohistochemistry-Paraffin: LARP1 Antibody [NBP1-19128] - Section of human colon carcinoma. Antibody:Affinity purified rabbit anti-LARP1 used at a dilution of 1:200 (1ug/ml). Detection: DABApplications for LARP1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID 25951186)
Immunohistochemistry-Paraffin
1:100 to 1:500
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: LARP1
Alternate Names
KIAA0731LARPMGC19556, La ribonucleoprotein domain family member 1, La ribonucleoprotein domain family, member 1, la-related protein 1
Entrez Gene IDs
23367 (Human)
Gene Symbol
LARP1
UniProt
Additional LARP1 Products
Product Documents for LARP1 Antibody
Product Specific Notices for LARP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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