Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all LASS6 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of the human LASS6. The immunogen is located within the last 50 amino acids of LASS6. Amino Acid Squence: DSEPPGKNPHTAT

Specificity

At least two isoforms of LASS6 are known to exist. This antibody is predicted not to cross-react with LASS5.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LASS6 Antibody - BSA Free

Western Blot: LASS6 AntibodyBSA Free [NBP1-76965]

Western Blot: LASS6 AntibodyBSA Free [NBP1-76965]

Western Blot: LASS6 Antibody [NBP1-76965] - Rat brain tissue lysate with LASS6 antibody at (A) 1 and (B) 2 ug/ml.
Knockdown Validated: LASS6 Antibody - BSA Free [NBP1-76965]

Western Blot: LASS6 Antibody - BSA Free [NBP1-76965] -

Western Blot: LASS6 Antibody [NBP1-76965] - CerS6 (LASS6)-dependent C16-ceramide generation mediates stress response to serum starvation. Serum withdrawal elevates CerS6 in cultured cells with concomitant increase of p53 and p21 while CerS6 siRNA silencing prevents the p53 and p21 accumulation in response to serum withdrawal. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-018-06650-y) licensed under a CC-BY license.
LASS6 Antibody - BSA Free

Immunohistochemistry: LASS6 Antibody - BSA Free [NBP1-76965] -

Immunohistochemistry: LASS6 Antibody - BSA Free [NBP1-76965] - Immunohistochemistry of LASS6 in mouse brain tissue with LASS6 antibody at 2.5 ug/mL.
View 1 more images

Applications for LASS6 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LASS6

The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. Like the highly homologous LASS5, LASS6 is also an endoplasmic reticulum, multi-pass membrane protein. LASS6 is also involved in the synthesis of C14, C16 and C18-ceramide, but shows a preference for unsaturated fatty acids. LASS6 is broadly expressed in a wide range of tissues and microarray data suggests that it may play a role in cancer differentiation and early embryonic development. At least two isoforms of LASS6 are known to exist. This antibody is predicted not to cross-react with LASS5.

Alternate Names

CerS6, LAG1 homolog, ceramide synthase 6, LAG1 longevity assurance homolog 6, LAG1 longevity assurance homolog 6 (S. cerevisiae), longevity assurance homolog 6, MGC129949, MGC129950

Gene Symbol

CERS6

UniProt

AAI09285 (Human)

Additional LASS6 Products

Product Documents for LASS6 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LASS6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.