LCAT Antibody (9C2U3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15692

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15692-100ul
NBP3-15692-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9C2U3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LCAT Antibodies »

Product Summary for LCAT Antibody (9C2U3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LCAT (P04180). MGPPGSPWQWVTLLLGLLLPPAAPFWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LCAT Antibody (9C2U3)

Western Blot: LCAT Antibody (9C2U3) [NBP3-15692]

Western Blot: LCAT Antibody (9C2U3) [NBP3-15692]

Western Blot: LCAT Antibody (9C2U3) [NBP3-15692] - Western blot analysis of extracts of Mouse plasma, using LCAT antibody (NBP3-15692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Western Blot: LCAT Antibody (9C2U3) [NBP3-15692]

Western Blot: LCAT Antibody (9C2U3) [NBP3-15692]

Western Blot: LCAT Antibody (9C2U3) [NBP3-15692] - Western blot analysis of extracts of Rat plasma, using LCAT antibody (NBP3-15692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for LCAT Antibody (9C2U3)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LCAT

Lecithin-Cholesterol Acyltransferase (LCAT) is the plasma enzyme responsible for most HDL cholesteryl esters in human plasma. In species with cholesteryl ester transfer protein (CETP) it is also responsible for a significant proportion of VLDL and LDL cholesteryl esters. LCAT, through the esterification of cholesterol, plays a role in the reverse cholesterol transport pathway by facilitating the net movement of cholesterol from peripheral tissues back to the liver for excretion. LCAT deficiency results in low levels of plasma HDL, whereas a transgenic overexpression of LCAT results in increased plasma HDL concentrations.

LCAT antibodies are useful tools for lipid and cholesterol metabolism research.

Alternate Names

EC 2.3.1.43, lecithin-cholesterol acyltransferasePhospholipid-cholesterol acyltransferase, phosphatidylcholine-sterol acyltransferase

Gene Symbol

LCAT

Additional LCAT Products

Product Documents for LCAT Antibody (9C2U3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LCAT Antibody (9C2U3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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