LCMT1 Antibody (OTI2C9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00923

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C9

Concentration

0.51 mg/ml

View all LCMT1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LCMT1(NP_057393) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LCMT1 Antibody (OTI2C9)

Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LCMT1 monoclonal antibody.
Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Immunohistochemistry-Paraffin: LCMT1 Antibody (OTI2C9) [NBP2-00923] - Staining of paraffin-embedded Human tonsil using anti-LCMT1 mouse monoclonal antibody.
Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923]

Western Blot: LCMT1 Antibody (OTI2C9) [NBP2-00923] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LCMT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LCMT1.
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Applications for LCMT1 Antibody (OTI2C9)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.51 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LCMT1

LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM].

Alternate Names

CGI-68, EC 2.1.1.-, LCMT, leucine carboxyl methyltransferase 1, PPMT1, protein phosphatase methyltransferase 1, Protein-leucine O-methyltransferase

Entrez Gene IDs

51451 (Human)

Gene Symbol

LCMT1

Additional LCMT1 Products

Product Documents for LCMT1 Antibody (OTI2C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LCMT1 Antibody (OTI2C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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