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LCMT2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76585

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human TYW4. The immunogen is located within the first 50 amino acids of TYW4. Amino Acid Squence: VQNTNDSSALSKRSLA

Specificity

TYW4 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW4 are known to exist; this antibody will detect only the long isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LCMT2 Antibody - BSA Free

Western Blot: LCMT2 AntibodyBSA Free [NBP1-76585]

Western Blot: LCMT2 AntibodyBSA Free [NBP1-76585]

Western Blot: LCMT2 Antibody [NBP1-76585] - Rat brain tissue lysate with LCMT2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: LCMT2 Antibody - BSA Free [NBP1-76585]

Immunocytochemistry/ Immunofluorescence: LCMT2 Antibody - BSA Free [NBP1-76585]

Immunocytochemistry/Immunofluorescence: LCMT2 Antibody [NBP1-76585] - Immunofluorescence of LCMT2 in mouse brain tissue with LCMT2 antibody at 20 ug/mL.
Immunohistochemistry: LCMT2 Antibody - BSA Free [NBP1-76585]

Immunohistochemistry: LCMT2 Antibody - BSA Free [NBP1-76585]

Immunohistochemistry: LCMT2 Antibody [NBP1-76585] - Immunohistochemistry of TYW4 in mouse brain tissue with TYW4 antibody at 5 ug/mL.

Applications for LCMT2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LCMT2

TYW4 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW4 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.

Alternate Names

EC 2.1.1.-, FLJ51321, KIAA0547tRNA-Wybutosine-synthesizing protein 4, leucine carboxyl methyltransferase 2, MGC9534, p21WAF1/CIP1 promoter-interacting protein, PPM2, tRNA wybutosine-synthesizing protein 4 homolog, tRNA yW-synthesizing protein 4 homolog, TYW4tRNA-yW synthesizing protein 4

Gene Symbol

LCMT2

Additional LCMT2 Products

Product Documents for LCMT2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LCMT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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