LEF1 Antibody (5A3)
Novus Biologicals, part of Bio-Techne | Catalog # H00051176-M03
![ELISA: LEF1 Antibody (5A3) [H00051176-M03]](https://resources.bio-techne.com/images/products/LEF1-Antibody-5A3-ELISA-H00051176-M03-img0004.jpg "ELISA: LEF1 Antibody (5A3) [H00051176-M03]")
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5A3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
LEF1 (NP_057353, 14 a.a. ~ 123 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DPELCATDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNESEIIPASNGHEVARQAQTSQEPYHDKAREHPDDGKHPDGGLYNKGPSYSSYSGYIMMPNMNND
Reactivity Notes
Human. Other species not tested.
Specificity
LEF1 (5A3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for LEF1 Antibody (5A3)
![Western Blot: LEF1 Antibody (5A3) [H00051176-M03]](https://resources.bio-techne.com/images/products/LEF1-Antibody-5A3-Western-Blot-H00051176-M03-img0007.jpg "Western Blot: LEF1 Antibody (5A3) [H00051176-M03]")
Western Blot: LEF1 Antibody (5A3) [H00051176-M03]
Western Blot: LEF1 Antibody (5A3) [H00051176-M03] - Analysis of LEF1 expression in HepG2 (Cat # L019V1).![Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (5A3) [H00051176-M03]](https://resources.bio-techne.com/images/products/LEF1-Antibody-5A3-Immunocytochemistry-Immunofluorescence-H00051176-M03-img0006.jpg "Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (5A3) [H00051176-M03]")
Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (5A3) [H00051176-M03]
Immunocytochemistry/Immunofluorescence: LEF1 Antibody (5A3) [H00051176-M03] - Analysis of monoclonal antibody to LEF1 on HeLa cell. Antibody concentration 10 ug/ml![Western Blot: LEF1 Antibody (5A3) [H00051176-M03]](https://resources.bio-techne.com/images/products/LEF1-Antibody-5A3-Western-Blot-H00051176-M03-img0008.jpg "Western Blot: LEF1 Antibody (5A3) [H00051176-M03]")
Western Blot: LEF1 Antibody (5A3) [H00051176-M03]
Western Blot: LEF1 Antibody (5A3) [H00051176-M03] - Analysis of LEF1 expression in Jurkat (Cat # L017V1).Applications for LEF1 Antibody (5A3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: LEF1
Long Name
Lymphoid Enhancer-binding Factor 1
Alternate Names
TCF10, TCF1ALPHA, TCF7L3
Entrez Gene IDs
51176 (Human)
Gene Symbol
LEF1
OMIM
153245 (Human)
UniProt
Additional LEF1 Products
Product Documents for LEF1 Antibody (5A3)
Product Specific Notices for LEF1 Antibody (5A3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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