Lefty-1 Antibody (C8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-41811

Novus Biologicals, part of Bio-Techne
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NBP3-41811
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # C8

Concentration

1 mg/ml

View all Lefty-1 Antibodies »

Product Specifications

Immunogen

Immunized with recombinant Human Lefty-1 (Uniprot#: O75610; Phe78-Pro361 (Uniprot#: O75610)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Lefty-1 Antibody (C8)

Lefty-1 Antibody (C8)

Immunohistochemistry-Paraffin: Lefty-1 Antibody (C8) [NBP3-41811] -

Immunohistochemistry-Paraffin: Lefty-1 Antibody (C8) [NBP3-41811] - DAB staining on IHC-P; Samples: Human Pancreas Tissue; Primary Ab: 30ug/ml NBP3-41811; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Applications for Lefty-1 Antibody (C8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-20ug/mL

Immunohistochemistry

5-30ug/mL

Western Blot

0.01-2ug/mL

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01M PBS, pH7.4, 50% glycerol

Preservative

0.05% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lefty-1

Lefty proteins are novel TGF-beta ligands that function as antagonists of Nodal signaling. The expression of Lefty proteins on the left side of the developing mouse embryo earned this protein family its name. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A and Lefty-B). By amino acid sequence, human Lefty-A and Lefty-B are more similar to each other (96%) than to either Lefty-1 or Lefty-2 in the mouse (81-82%). The Leftys contains some of the features of the cysteine-knot structure conserved among TGF-beta-related proteins, but also some structural differences. Specifically, they lack the alpha-helical segment important for ligand dimerization as well as the cysteine residue involved in stabilization of dimers.

Alternate Names

Leftb, Lefty-B, Lefty1

Gene Symbol

LEFTY1

Additional Lefty-1 Products

Product Documents for Lefty-1 Antibody (C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lefty-1 Antibody (C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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