Lefty1/2 Antibody (2Y10P2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16118
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2Y10P2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 267-366 of human Lefty1/2 (O75610). EMYIDLQGMKWAENWVLEPPGFLAYECVGTCRQPPEALAFKWPFLGPRQCIASETDSLPMIVSIKEGGRTRPQVVSLPNMRVQKCSCASDGALVPRRLQP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Lefty1/2 Antibody (2Y10P2)
![Western Blot: Lefty1/2 Antibody (2Y10P2) [NBP3-16118]](https://resources.bio-techne.com/images/products/Lefty1-2-Antibody-2Y10P2-Western-Blot-NBP3-16118-img0002.jpg "Western Blot: Lefty1/2 Antibody (2Y10P2) [NBP3-16118]")
Western Blot: Lefty1/2 Antibody (2Y10P2) [NBP3-16118]
Western Blot: Lefty1/2 Antibody (2Y10P2) [NBP3-16118] - Western blot analysis of extracts of various cell lines, using Lefty1/2 Rabbit mAb (NBP3-16118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunohistochemistry-Paraffin: Lefty1/2 Antibody (2Y10P2) [NBP3-16118]](https://resources.bio-techne.com/images/products/Lefty1-2-Antibody-2Y10P2-Immunohistochemistry-Paraffin-NBP3-16118-img0001.jpg "Immunohistochemistry-Paraffin: Lefty1/2 Antibody (2Y10P2) [NBP3-16118]")
Immunohistochemistry-Paraffin: Lefty1/2 Antibody (2Y10P2) [NBP3-16118]
Immunohistochemistry-Paraffin: Lefty1/2 Antibody (2Y10P2) [NBP3-16118] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Lefty1/2 Rabbit mAb (NBP3-16118) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.![Western Blot: Lefty1/2 Antibody (2Y10P2) [NBP3-16118]](https://resources.bio-techne.com/images/products/Lefty1-2-Antibody-2Y10P2-Western-Blot-NBP3-16118-img0003.jpg "Western Blot: Lefty1/2 Antibody (2Y10P2) [NBP3-16118]")
Western Blot: Lefty1/2 Antibody (2Y10P2) [NBP3-16118]
Western Blot: Lefty1/2 Antibody (2Y10P2) [NBP3-16118] - Western blot analysis of extracts of various cell lines, using Lefty1/2 Rabbit mAb (NBP3-16118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Applications for Lefty1/2 Antibody (2Y10P2)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Lefty1/2
Alternate Names
Beta-4 (Endometrial Bleeding-Associated Factor; LEFTY A), EBAF, endometrial bleeding associated factor (left-right determination, Endometrial Bleeding Associated Factor (Left-Right Determination, Factor A;, Endometrial Bleeding-Associated Factor, LEFTA, Left-Right Determination Factor 2, Left-Right Determination Factor A, LEFTY2, LEFTYA, Protein Lefty-2, Protein Lefty-A, TGFB4, TGF-beta-4, Transforming Growth Factor, Transforming Growth Factor Beta-4
Gene Symbol
LEFTY2
Additional Lefty1/2 Products
Product Documents for Lefty1/2 Antibody (2Y10P2)
Product Specific Notices for Lefty1/2 Antibody (2Y10P2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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