Legumain/Asparaginyl Endopeptidase Antibody (JE35-56)

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (JE35-56) [NBP3-32528]

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (JE35-56) [NBP3-32528] - Western blot analysis of Legumain/Asparaginyl Endopeptidase on different lysates with Rabbit anti-Legumain/Asparaginyl Endopeptidase antibody (NBP3-32528) at 1/1,000 dilution.

Lane 1: HT-29 cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: 293T cell lysate (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: Neuro-2a cell lysate (20 ug/Lane)
Lane 6: MCF7 cell lysate (20 ug/Lane)
Lane 7: RAW264.7 cell lysate (20 ug/Lane)
Lane 8: Mouse kidney tissue lysate (40 ug/Lane)
Lane 9: Mouse placenta tissue lysate (40 ug/Lane)
Lane 10: Rat kidney tissue lysate (40 ug/Lane)
Lane 11: Human kidney tissue lysate (40 ug/Lane)

Predicted band size: 49 kDa
Observed band size: 36/46/56 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32528) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Legumain/Asparaginyl Endopeptidase Antibody (JE35-56) [NBP3-32528]

Immunohistochemistry: Legumain/Asparaginyl Endopeptidase Antibody (JE35-56) [NBP3-32528] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Legumain/Asparaginyl Endopeptidase antibody (NBP3-32528) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32528) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.