Legumain/Asparaginyl Endopeptidase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87793

Novus Biologicals, part of Bio-Techne
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NBP1-87793
NBP1-87793-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Legumain/Asparaginyl Endopeptidase Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Legumain/Asparaginyl Endopeptidase Antibody

Immunocytochemistry/ Immunofluorescence: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793]

Immunocytochemistry/ Immunofluorescence: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793]

Immunocytochemistry/Immunofluorescence: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793] - Staining of human cell line RT4 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793]

Immunohistochemistry-Paraffin: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793]

Immunohistochemistry-Paraffin: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793]

Immunohistochemistry-Paraffin: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793]

Immunohistochemistry-Paraffin: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793] - Staining of human tonsil shows strong granular cytoplasmic positivity in non-germinal center cells.
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Applications for Legumain/Asparaginyl Endopeptidase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Legumain/Asparaginyl Endopeptidase

Legumain encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq]

Alternate Names

AEP, Asparaginyl Endopeptidase, LGMN

Gene Symbol

LGMN

Additional Legumain/Asparaginyl Endopeptidase Products

Product Documents for Legumain/Asparaginyl Endopeptidase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Legumain/Asparaginyl Endopeptidase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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