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LEMD3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81265

Novus Biologicals, part of Bio-Techne
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NBP1-81265
NBP1-81265-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QGQAFHLDRRNSPPNSLTPCLKIRNMFDPVMEIGDQWHLAIQEAILEKCSDNDGIVHIAVDKNSREGCVYVKCLSPEYAGKAFKALHGSWFDGKLVTVKYLRLDRYHHRFPQALTSNTPLKPSNKHMNSMSHLRLRT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LEMD3 Antibody

Immunohistochemistry-Paraffin: LEMD3 Antibody [NBP1-81265]

Immunohistochemistry-Paraffin: LEMD3 Antibody [NBP1-81265]

Immunohistochemistry-Paraffin: LEMD3 Antibody [NBP1-81265] - Staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.
LEMD3 Antibody Western Blot: LEMD3 Antibody Antibody [NBP1-81265]

Western Blot: LEMD3 Antibody Antibody [NBP1-81265]

Analysis using NBP1-81265 (A) shows similar pattern to independent antibody NBP2-57649 (B).

Applications for LEMD3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LEMD3

The MAN antigens, such as MAN1, are nuclear membrane proteins that are recognized by autoantibodies from a patient with a collagen vascular disease. Because MAN1 contains an LEM domain, the gene is also called LEMD3 (for 'LEM domain-containing-3').[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

inner nuclear membrane protein Man1, integral inner nuclear membrane protein, LEM domain containing 3, MAN1LEM domain-containing protein 3

Gene Symbol

LEMD3

Additional LEMD3 Products

Product Documents for LEMD3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LEMD3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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