LEO1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89423

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-89423
NBP1-89423-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LEO1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SGQPSNKELFGDDSEDEGASHHSGSDNHSERSDNRSEASERSDHEDNDPSDVDQHSGSEAPNDDEDEGHRSDGGSHHSEAEGSEKAHSDDEKWGREDKSDQSDDEKIQNSD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LEO1 Antibody

Western Blot: LEO1 Antibody [NBP1-89423]

Western Blot: LEO1 Antibody [NBP1-89423]

Western Blot: LEO1 Antibody [NBP1-89423] - Analysis in control (vector only transfected HEK293T lysate) and LEO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: LEO1 Antibody [NBP1-89423]

Immunocytochemistry/ Immunofluorescence: LEO1 Antibody [NBP1-89423]

Immunocytochemistry/Immunofluorescence: LEO1 Antibody [NBP1-89423] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center & centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LEO1 Antibody [NBP1-89423]

Immunohistochemistry-Paraffin: LEO1 Antibody [NBP1-89423]

Immunohistochemistry-Paraffin: LEO1 Antibody [NBP1-89423] - Staining of human rectum shows nuclear and cytoplasmic positivity in glandular cells.

Applications for LEO1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LEO1

Leo1 is one of four subunits of the Paf complex, which has been shown to aid in the regulation of transcriptional elongation, histone methylation and RNA processing in yeast.

Alternate Names

Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae), RDLReplicative senescence down-regulated leo1-like protein, RNA polymerase-associated protein LEO1

Gene Symbol

LEO1

Additional LEO1 Products

Product Documents for LEO1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LEO1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.