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LETM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89556

Novus Biologicals, part of Bio-Techne
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NBP1-89556
NBP1-89556-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SKTGEEKYVEESKASKRLTKRVQQMIGQIDGLISQLEMDQQAGKLAPANGMPTGENVISVAELINAMKQVKHIPESKLTSLAAALDENKDGKVNIDDLVKVIELVDKEDVHISTSQVAEIVATLE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LETM1 Antibody

Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-89556]

Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-89556]

Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-89556] - Staining in human colon and pancreas tissues using anti-LETM1 antibody. Corresponding LETM1 RNA-seq data are presented for the same tissues.
Western Blot: LETM1 Antibody [NBP1-89556]

Western Blot: LETM1 Antibody [NBP1-89556]

Western Blot: LETM1 Antibody [NBP1-89556] - Analysis in human cell line MOLT-4.
Immunocytochemistry/ Immunofluorescence: LETM1 Antibody [NBP1-89556]

Immunocytochemistry/ Immunofluorescence: LETM1 Antibody [NBP1-89556]

Immunocytochemistry/Immunofluorescence: LETM1 Antibody [NBP1-89556] - Staining of human cell line U-251 MG shows localization to mitochondria.

Applications for LETM1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LETM1

The LETM1 gene encodes a protein that is localized to the inner mitochondrial membrane. The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. Mutations in this gene cause

Alternate Names

LETM1 and EF-hand domain-containing protein 1, mitochondrial, leucine zipper-EF-hand containing transmembrane protein 1, Leucine zipper-EF-hand-containing transmembrane protein 1, Mdm38 homolog

Gene Symbol

LETM1

Additional LETM1 Products

Product Documents for LETM1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LETM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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