Leukotriene A4 Hydrolase/LTA4H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99029

Novus Biologicals, part of Bio-Techne
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NBP2-99029-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Leukotriene A4 Hydrolase/LTA4H Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Leukotriene A4 Hydrolase/LTA4H (Accession#: NP_000886.1; Met 1-Asp 611)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Leukotriene A4 Hydrolase/LTA4H Antibody

Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029]

Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029]

Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 63 kDa (We are unsure as to the identity of these extra bands).
Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029]

Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029]

Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 63 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029]

Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029]

Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Leukotriene A4 Hydrolase/LTA4H was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 2 ul anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 69 kDa

Applications for Leukotriene A4 Hydrolase/LTA4H Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

2-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Leukotriene A4 Hydrolase/LTA4H

Hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). The enzyme also has some peptidase activity

Alternate Names

LTA4H

Gene Symbol

LTA4H

Additional Leukotriene A4 Hydrolase/LTA4H Products

Product Documents for Leukotriene A4 Hydrolase/LTA4H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Leukotriene A4 Hydrolase/LTA4H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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