Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7LE

Format

BSA Free

Concentration

1.0 mg/ml

View all Lewis A Blood Group Antigen Antibodies »

Product Specifications

Immunogen

Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).

Specificity

The antibody 7LE recognizes Lewisa blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewisa may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Lewis A Blood Group Antigen Antibody (7LE) - BSA Free

Immunohistochemistry: Lewis A Blood Group Antigen Antibody (7LE) - BSA Free [NB500-525]

Immunohistochemistry: Lewis A Blood Group Antigen Antibody (7LE) - BSA Free [NB500-525]

Immunohistochemistry: Lewis A Blood Group Antigen Antibody (7LE) [NB500-525] - Staining of human colon adenocarcinoma (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE).
Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (7LE) - BSA Free [NB500-525]

Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (7LE) - BSA Free [NB500-525]

Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (7LE) [NB500-525] - Staining of colorectal adenocarcinoma with anti-FUT3/Blood Group Lewis A (7LE).
Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (7LE) - BSA Free [NB500-525]

Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (7LE) - BSA Free [NB500-525]

Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (7LE) [NB500-525] - Staining of human pancreas (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE).

Applications for Lewis A Blood Group Antigen Antibody (7LE) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1:1000
Application Notes
Purity is > 95% (by SDS-PAGE). For IHC-P use heat mediated antigen retrieval (sodium citrate).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lewis A Blood Group Antigen

This antibody recognizes FUT3/Blood Group Lewis A, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. FUT3/Blood Group Lewis A may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.

Alternate Names

A transferase, A3GALNT, A3GALT1, ABO, B transferase, EC=2.4.1.37, EC=2.4.1.40, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, GTB, Histo-blood group A transferase, Histo-blood group ABO system transferase, Histo-blood group B transferase, NAGAT

Entrez Gene IDs

2525 (Human)

Gene Symbol

FUT3

UniProt

P21217

Additional Lewis A Blood Group Antigen Products

Product Documents for Lewis A Blood Group Antigen Antibody (7LE) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lewis A Blood Group Antigen Antibody (7LE) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.