Lewis A Blood Group Antigen Antibody (SPM279) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48079
![Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (SPM279) - IHC-Prediluted [NBP2-48079]](https://resources.bio-techne.com/images/products/Lewis-A-Blood-Group-Antigen-Antibody-SPM279-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48079-img0001.jpg "Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (SPM279) - IHC-Prediluted [NBP2-48079]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM279
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Lewis A Blood Group Antigen Antibody (SPM279) - IHC-Prediluted
Immunogen
Mucins isolated from ovarian cyst fluid
Localization
Cell membrane, cytoplasmic and extracellular
Specificity
Recognizes a carbohydrate determinant of Gal 1-3(Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Lewis A Blood Group Antigen Antibody (SPM279) - IHC-Prediluted
Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (SPM279) - IHC-Prediluted [NBP2-48079]
Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (SPM279) - IHC-Prediluted [NBP2-48079] - Human Colon Carcinoma stained with Lewis A Monoclonal Antibody (SPM279).Applications for Lewis A Blood Group Antigen Antibody (SPM279) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Lewis A Blood Group Antigen
Alternate Names
A transferase, A3GALNT, A3GALT1, ABO, B transferase, EC=2.4.1.37, EC=2.4.1.40, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, GTB, Histo-blood group A transferase, Histo-blood group ABO system transferase, Histo-blood group B transferase, NAGAT
Gene Symbol
ABO
Additional Lewis A Blood Group Antigen Products
Product Documents for Lewis A Blood Group Antigen Antibody (SPM279) - IHC-Prediluted
Product Specific Notices for Lewis A Blood Group Antigen Antibody (SPM279) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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