Lewis Y Antibody (A70-A/A9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50073
![Immunohistochemistry-Paraffin: Lewis Y Antibody (A70-A/A9) [NBP2-50073]](https://resources.bio-techne.com/images/products/Lewis-Y-Antibody-A70-A-A9-Immunohistochemistry-Paraffin-NBP2-50073-img0001.jpg "Immunohistochemistry-Paraffin: Lewis Y Antibody (A70-A/A9) [NBP2-50073]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # A70-A/A9
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Live Ls174T cells (human colon carcinoma cell line)
Epitope
(Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-
Localization
Cell membrane, cytoplasmic and extracellular
Specificity
This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis Y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-). Lewis Y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis Y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Marker
Tumor Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54561)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Lewis Y Antibody (A70-A/A9)
Immunohistochemistry-Paraffin: Lewis Y Antibody (A70-A/A9) [NBP2-50073]
Immunohistochemistry-Paraffin: Lewis Y Antibody (A70-A/A9) [NBP2-50073] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Lewis Y Monoclonal Antibody (A70-A/A9).Applications for Lewis Y Antibody (A70-A/A9)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Lewis Y
Alternate Names
IPCA-2, IPCA6, IPCA-6, IPCA-8, PCANAP2, PCANAP6, PCANAP8, Prostate cancer-associated protein 6, PRST, Solute carrier family 45 member 3
Additional Lewis Y Products
Product Documents for Lewis Y Antibody (A70-A/A9)
Product Specific Notices for Lewis Y Antibody (A70-A/A9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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