Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LWY/1463

Concentration

0.2 mg/ml

View all Lewis Y Antibodies »

Product Specifications

Immunogen

Human colon carcinoma cells

Epitope

(Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-

Localization

Cell membrane, Cytoplasmic and extracellular

Specificity

This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis Y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-). Lewis Y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis Y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.

Marker

Tumor Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54560)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Lewis Y Antibody (LWY/1463)

Immunohistochemistry-Paraffin: Lewis Y Antibody (LWY/1463) [NBP2-53216]

Immunohistochemistry-Paraffin: Lewis Y Antibody (LWY/1463) [NBP2-53216]

Immunohistochemistry-Paraffin: Lewis Y Antibody (LWY/1463) [NBP2-53216] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Lewis Y Monoclonal Antibody (LWY/1463).

Applications for Lewis Y Antibody (LWY/1463)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Lewis Y

Alternate Names

IPCA-2, IPCA6, IPCA-6, IPCA-8, PCANAP2, PCANAP6, PCANAP8, Prostate cancer-associated protein 6, PRST, Solute carrier family 45 member 3

Additional Lewis Y Products

Product Documents for Lewis Y Antibody (LWY/1463)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lewis Y Antibody (LWY/1463)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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