Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all LGTN Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 350 and 400 of human eIF2D using the numbering given in entry NP_008824.2 (GeneID 1939).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LGTN Antibody

Western Blot: LGTN Antibody [NBP1-50038]

Western Blot: LGTN Antibody [NBP1-50038]

Western Blot: LGTN Antibody [NBP1-50038] - Detection of Human Ligatin by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Ligatin antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Ligatin was also immunoprecipitated by rabbit anti-Ligatin antibody BL10965, which recognizes a downstream epitope. For blotting immunoprecipitated Ligatin, the ReliaBLOT(R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for LGTN Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LGTN

This gene encodes a protein receptor that localizes phosphoglycoproteins within endosomes and at the cell periphery. This trafficking receptor for phosphoglycoproteins may play a role in neuroplasticity by modulating cell-cell interactions, intracellular adhesion, and protein binding at membrane surfaces. In hippocampal neurons, long-lasting down-regulation of ligatin mRNA levels occurs via post-transcriptional RNA processing following glutamate receptor activation. This protein contains single PUA and SUI1 domains and these domains may function in RNA binding and translation initiation, respectively.

Alternate Names

eukaryotic translation initiation factor 2D, HCA56eIF2d, Hepatocellular carcinoma-associated antigen 56, LGTNLigatin, ligatin

Gene Symbol

EIF2D

Additional LGTN Products

Product Documents for LGTN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LGTN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.