LHX2 Antibody (JE54-99)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32533
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE54-99
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human LHX2 aa 1-100 / 406. (Uniprot: P50458)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for LHX2 Antibody (JE54-99)
Western Blot: LHX2 Antibody (JE54-99) [NBP3-32533]
Western Blot: LHX2 Antibody (JE54-99) [NBP3-32533] - Western blot analysis of LHX2 on different lysates with Rabbit anti-LHX2 antibody (NBP3-32533) at 1/1,000 dilution.Lane 1: A431 cell lysate (20 ug/Lane)
Lane 2: NIH/3T3 cell lysate (20 ug/Lane)
Lane 3: Neuro-2a cell lysate (20 ug/Lane)
Lane 4: Human brain tissue lysate (40 ug/Lane)
Lane 5: Mouse brain tissue lysate (40 ug/Lane)
Lane 6: Rat brain tissue lysate (40 ug/Lane)
Predicted band size: 44 kDa
Observed band size: 40-44 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32533) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for LHX2 Antibody (JE54-99)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LHX2
Alternate Names
hLhx2, Homeobox protein LH-2, LH-2, LH2MGC138390, LIM homeobox 2, LIM homeobox protein 2, LIM HOX gene 2, LIM/homeobox protein Lhx2
Gene Symbol
LHX2
Additional LHX2 Products
Product Documents for LHX2 Antibody (JE54-99)
Product Specific Notices for LHX2 Antibody (JE54-99)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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