LHX3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13352

Novus Biologicals, part of Bio-Techne
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NBP3-13352
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LHX3 Antibodies »

Product Summary for LHX3 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse LHX3. The exact sequence is proprietary.

Reactivity Notes

Human (83%), Pig (82%), Bovine (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for LHX3 Antibody

Western Blot: LHX3 Antibody [NBP3-13352]

Western Blot: LHX3 Antibody [NBP3-13352]

Western Blot: LHX3 Antibody [NBP3-13352] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with LHX3 antibody (NBP3-13352) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: LHX3 Antibody [NBP3-13352]

Immunocytochemistry/ Immunofluorescence: LHX3 Antibody [NBP3-13352]

Immunocytochemistry/Immunofluorescence: LHX3 Antibody [NBP3-13352] - LHX3 antibody detects LHX3 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LHX3 stained by LHX3 antibody (NBP3-13352) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: LHX3 Antibody [NBP3-13352]

Immunohistochemistry-Paraffin: LHX3 Antibody [NBP3-13352]

Immunohistochemistry-Paraffin: LHX3 Antibody [NBP3-13352] - LHX3 antibody detects LHX3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E10.5 embryo. Green: LHX3 stained by LHX3 antibody (NBP3-13352) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for LHX3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
ICC/IF-Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LHX3

The LHX3 gene encodes a protien of that has a unique cyesteine-rich zinc-binding domain. This protien is a required transciption factor in pituitary development as well as motor neuron specification.

Alternate Names

CPHD3, DKFZp762A2013, LIM homeobox 3, LIM homeobox protein 3, LIM/homeobox protein Lhx3, LIM/homeodomain protein LHX3, LIM3, M2-LHX3

Gene Symbol

LHX3

Additional LHX3 Products

Product Documents for LHX3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LHX3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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