LIAS Antibody (PSH04-04)

Western Blot: LIAS Antibody (PSH04-04) [NBP3-32536]

Western Blot: LIAS Antibody (PSH04-04) [NBP3-32536] - Western blot analysis of LIAS on different lysates with Rabbit anti-LIAS antibody (NBP3-32536) at 1/2,000 dilution.

Lane 1: K-562 cell lysate (20 ug/Lane)
Lane 2: LO2 cell lysate (20 ug/Lane)
Lane 3: Raji cell lysate (20 ug/Lane)
Lane 4: MCF7 cell lysate (20 ug/Lane)
Lane 5: Jurkat cell lysate (20 ug/Lane)
Lane 6: HepG2 cell lysate (20 ug/Lane)
Lane 7: PC-3M cell lysate (20 ug/Lane)
Lane 8: C2C12 cell lysate (20 ug/Lane)
Lane 9: L6 cell lysate (20 ug/Lane)
Lane 10: Mouse liver tissue lysate (40 ug/Lane)
Lane 11: Mouse colon tissue lysate (40 ug/Lane)
Lane 12: Mouse brain tissue lysate (40 ug/Lane)
Lane 13: Rat liver tissue lysate (40 ug/Lane)
Lane 14: Rat testis tissue lysate (40 ug/Lane)

Predicted band size: 42 kDa
Observed band size: 39 kDa

Exposure time: 42 seconds; ECL;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32536) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: LIAS Antibody (PSH04-04) [NBP3-32536]

Immunocytochemistry/ Immunofluorescence: LIAS Antibody (PSH04-04) [NBP3-32536] - Immunocytochemistry analysis of C2C12 cells labeling LIAS with Rabbit anti-LIAS antibody (NBP3-32536) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIAS antibody (NBP3-32536) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.