LIM Kinase 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23404

Novus Biologicals, part of Bio-Techne
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NBP3-23404
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LIM Kinase 1 Antibodies »

Product Summary for LIM Kinase 1 Antibody

Immunogen

LIM Kinase 1 (C-terminus) synthetic peptide (coupled to carrier protein) corresponding to amino acids at the C-terminus of human LIM Kinase 1. This sequence is conserved in rat and mouse LIM Kinase 1, and is not found in LIM Kinase 2. [UniProt# P53667]

Specificity

The antibody detects a 72 kDa protein corresponding to the molecular mass of LIM Kinase 1 on SDS-PAGE immunoblots of mouse recombinant LIM Kinase 1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for LIM Kinase 1 Antibody

LIM Kinase 1 Antibody

Western Blot: LIM Kinase 1 Antibody [NBP3-23404] -

Western Blot: LIM Kinase 1 Antibody [NBP3-23404] - Western blot image of activated mouse recombinant LIM Kinase 1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-LIM Kinase 1 (C-term.) (lanes 1 & 2) and anti-LIM Kinase 1 (Thr-508) (lanes 3 & 4).

Applications for LIM Kinase 1 Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: LIM Kinase 1

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.

Long Name

LIM domain kinase 1

Alternate Names

LIMK, LIMK-1, LIMK1

Gene Symbol

LIMK1

Additional LIM Kinase 1 Products

Product Documents for LIM Kinase 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LIM Kinase 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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