LIM kinase 2 Antibody (2H2-E11)
Novus Biologicals, part of Bio-Techne | Catalog # H00003985-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2H2-E11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
LIMK2 (AAH13051.1, 1 a.a. ~ 686 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSYLSVPAYFTSRDLFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRAPPGAAGEGPGCADDEGPVRRQGKVTIKYDPKELRKHLNLEEWILEQLTRLYDCQEEEISELEIDVDELLDMESDDAWASRVKELLVDCYKPTEAFISGLLDKIRAMQKLSTPQKK
Specificity
LIMK2 - LIM domain kinase 2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for LIM kinase 2 Antibody (2H2-E11)
Western Blot: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01]
Western Blot: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01] - Analysis of LIMK2 expression in transfected 293T cell line by LIMK2 monoclonal antibody (M01), clone 2H2-E11.Lane 1: LIMK2 transfected lysate(72.232 KDa).Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01]
Immunocytochemistry/Immunofluorescence: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01] - Analysis of monoclonal antibody to LIMK2 on HeLa cell . Antibody concentration 10 ug/ml.ELISA: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01]
ELISA: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01] - Detection limit for recombinant GST tagged LIMK2 is approximately 0.3ng/ml as a capture antibody.Applications for LIM kinase 2 Antibody (2H2-E11)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: LIM kinase 2
Long Name
LIM domain kinase 2
Alternate Names
LIMK-2, LIMK2
Entrez Gene IDs
3985 (Human)
Gene Symbol
LIMK2
OMIM
601988 (Human)
UniProt
Additional LIM kinase 2 Products
Product Documents for LIM kinase 2 Antibody (2H2-E11)
Product Specific Notices for LIM kinase 2 Antibody (2H2-E11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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