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LIM kinase 2 Antibody (2H2-E11)

Novus Biologicals, part of Bio-Techne | Catalog # H00003985-M01

Novus Biologicals, part of Bio-Techne
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H00003985-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2H2-E11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

LIMK2 (AAH13051.1, 1 a.a. ~ 686 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSYLSVPAYFTSRDLFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRAPPGAAGEGPGCADDEGPVRRQGKVTIKYDPKELRKHLNLEEWILEQLTRLYDCQEEEISELEIDVDELLDMESDDAWASRVKELLVDCYKPTEAFISGLLDKIRAMQKLSTPQKK

Specificity

LIMK2 - LIM domain kinase 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for LIM kinase 2 Antibody (2H2-E11)

Western Blot: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01]

Western Blot: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01]

Western Blot: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01] - Analysis of LIMK2 expression in transfected 293T cell line by LIMK2 monoclonal antibody (M01), clone 2H2-E11.Lane 1: LIMK2 transfected lysate(72.232 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01]

Immunocytochemistry/ Immunofluorescence: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01]

Immunocytochemistry/Immunofluorescence: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01] - Analysis of monoclonal antibody to LIMK2 on HeLa cell . Antibody concentration 10 ug/ml.
ELISA: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01]

ELISA: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01]

ELISA: LIM kinase 2 Antibody (2H2-E11) [H00003985-M01] - Detection limit for recombinant GST tagged LIMK2 is approximately 0.3ng/ml as a capture antibody.

Applications for LIM kinase 2 Antibody (2H2-E11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LIM kinase 2

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.

Long Name

LIM domain kinase 2

Alternate Names

LIMK-2, LIMK2

Entrez Gene IDs

3985 (Human)

Gene Symbol

LIMK2

OMIM

601988 (Human)

Additional LIM kinase 2 Products

Product Documents for LIM kinase 2 Antibody (2H2-E11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LIM kinase 2 Antibody (2H2-E11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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