LIM1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12886
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (99%), Chimpanzee (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for LIM1 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human LIM1. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (88%), Chicken (84%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for LIM1 Antibody
Western Blot: LIM1 Antibody [NBP3-12886]
Western Blot: LIM1 Antibody [NBP3-12886] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with LIM1 antibody (NBP3-12886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunohistochemistry-Paraffin: LIM1 Antibody [NBP3-12886]
Immunohistochemistry-Paraffin: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. LIM1 antibody (NBP3-12886) dilution: 1:500. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15minImmunohistochemistry-Frozen: LIM1 Antibody [NBP3-12886]
Immunohistochemistry-Frozen: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: LIM1 protein stained by LIM1 antibody (NBP3-12886) diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.Applications for LIM1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LIM1
Long Name
LIM Homeobox 1
Alternate Names
LHX1
Gene Symbol
LHX1
Additional LIM1 Products
Product Specific Notices for LIM1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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