LIME Antibody (LIME-06) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44945
Key Product Details
Species Reactivity
Human
Applications
Flow (Intracellular), Flow Cytometry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # LIME-06
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
Specificity
The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for LIME Antibody (LIME-06) - BSA Free
![Immunoprecipitation: LIME Antibody (LIME-06) - BSA Free [NBP1-44945]](https://resources.bio-techne.com/images/products/LIME-Antibody-LIME-06-Immunoprecipitation-NBP1-44945-img0003.jpg "Immunoprecipitation: LIME Antibody (LIME-06) - BSA Free [NBP1-44945]")
Immunoprecipitation: LIME Antibody (LIME-06) - BSA Free [NBP1-44945]
Immunoprecipitation: LIME Antibody (LIME-06) [NBP1-44945] - Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92; cat. no. NB500-521) or anti-human CD4 (MEM-16), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).Applications for LIME Antibody (LIME-06) - BSA Free
Application
Recommended Usage
Flow (Intracellular)
8-12 ug/ml
Immunoprecipitation
1:50
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: LIME
Alternate Names
dJ583P15.4, FLJ20406, Lck interacting transmembrane adaptor 1, Lck-interacting membrane protein, Lck-interacting molecule, lck-interacting transmembrane adapter 1, LIMELP8067
Gene Symbol
LIME1
Additional LIME Products
Product Documents for LIME Antibody (LIME-06) - BSA Free
Product Specific Notices for LIME Antibody (LIME-06) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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