Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # LIME-10

Format

BSA Free

Concentration

1.0 mg/ml

View all LIME Antibodies »

Product Specifications

Immunogen

COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.

Specificity

The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LIME Antibody (LIME-10) - BSA Free

LIME Antibody (LIME-10) - BSA Free Western Blot: LIME Antibody (LIME-10) - BSA Free [NB500-343]

Western Blot: LIME Antibody (LIME-10) - BSA Free [NB500-343]

Analysis of human LIME using mouse monoclonal antibody LIME-10 on lysates of J77-LIME transfectants and Jurkat cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 1 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LIME was detected around 30 kDa.
LIME Antibody (LIME-10) - BSA Free

Flow Cytometry: LIME Antibody (LIME-10) - BSA Free [NB500-343] -

Flow Cytometry: LIME Antibody (LIME-10) - BSA Free [NB500-343] - Anti-Hu LIME1 purified antibody (clone LIME-10) (concentration in sample 1 μg/ml, red-filled histogram) binds specifically to intacellular LIME1 in transfected cells (upper panel), but not to Jurkat cells (lower panel).Level of non-specific binding was assessed using Mouse IgG1 isotype control purified antibody (MOPC-21) under same conditions (concentration in sample 1 μg/ml, black-dashed histogram).

Applications for LIME Antibody (LIME-10) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-5 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

Reducing conditions; 1ug/ml
Application Notes
Western Blot: Predicted molecular weight 32.3 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LIME

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

Alternate Names

dJ583P15.4, FLJ20406, Lck interacting transmembrane adaptor 1, Lck-interacting membrane protein, Lck-interacting molecule, lck-interacting transmembrane adapter 1, LIMELP8067

Entrez Gene IDs

54923 (Human)

Gene Symbol

LIME1

Additional LIME Products

Product Documents for LIME Antibody (LIME-10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LIME Antibody (LIME-10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.