Key Product Details

Species Reactivity

Mouse

Applications

Immunoprecipitation, Western Blot

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # mLIME-05

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LIME Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to amino acids 86-202 of mouse LIME

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for LIME Antibody (mLIME-05) [DyLight 650]

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: LIME

LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.

Alternate Names

dJ583P15.4, FLJ20406, Lck interacting transmembrane adaptor 1, Lck-interacting membrane protein, Lck-interacting molecule, lck-interacting transmembrane adapter 1, LIMELP8067

Gene Symbol

LIME1

Additional LIME Products

Product Documents for LIME Antibody (mLIME-05) [DyLight 650]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LIME Antibody (mLIME-05) [DyLight 650]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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