Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5D3

Concentration

1 mg/ml

View all LIME Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 30-295 of human LIME1(NP_060276) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

31.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LIME Antibody (OTI5D3)

Western Blot: LIME Antibody (OTI5D3) [NBP2-45653]

Western Blot: LIME Antibody (OTI5D3) [NBP2-45653]

Western Blot: LIME Antibody (5D3) [NBP2-45653] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIME1.
Immunohistochemistry: LIME Antibody (OTI5D3) [NBP2-45653]

Immunohistochemistry: LIME Antibody (OTI5D3) [NBP2-45653]

Immunohistochemistry: LIME Antibody (5D3) [NBP2-45653] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LIME Antibody (OTI5D3) [NBP2-45653]

Immunohistochemistry: LIME Antibody (OTI5D3) [NBP2-45653]

Immunohistochemistry: LIME Antibody (5D3) [NBP2-45653] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for LIME Antibody (OTI5D3)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIME

LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.

Alternate Names

dJ583P15.4, FLJ20406, Lck interacting transmembrane adaptor 1, Lck-interacting membrane protein, Lck-interacting molecule, lck-interacting transmembrane adapter 1, LIMELP8067

Gene Symbol

LIME1

Additional LIME Products

Product Documents for LIME Antibody (OTI5D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LIME Antibody (OTI5D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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