LIME Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1436

Novus Biologicals, part of Bio-Techne
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NB100-1436
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all LIME Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ENVYESIKEMGL corresponding to C-Terminus according to Uniprot Mouse NP_076173.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LIME Antibody

Western Blot: LIME Antibody [NB100-1436]

Western Blot: LIME Antibody [NB100-1436]

Western Blot: LIME Antibody [NB100-1436] - (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for LIME Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 32 kDa band observed in mouse heart lysates (predicted MW of 31 kDa band according to NP_076173.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIME

LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.

Alternate Names

dJ583P15.4, FLJ20406, Lck interacting transmembrane adaptor 1, Lck-interacting membrane protein, Lck-interacting molecule, lck-interacting transmembrane adapter 1, LIMELP8067

Entrez Gene IDs

54923 (Human); 72699 (Mouse); 362289 (Rat)

Gene Symbol

LIME1

UniProt

NP_076173.1 (Mouse)

Additional LIME Products

Product Documents for LIME Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LIME Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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