LIMPII/SR-B2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB400-102

Novus Biologicals, part of Bio-Techne
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NB400-102
NB400-102SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Chinese Hamster, Hamster, Mustelid

Cited:

Human, Mouse, Rat, Hamster - Cricetulus (Chinese Hamster), Mustelid

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all LIMPII/SR-B2 Antibodies »

Product Specifications

Immunogen

A peptide containing residues from mouse SR-BII (between residues 400-478) plus an N-terminal cysteine was coupled to KLH. [UniProt# O35114]

Reactivity Notes

Mink.

Marker

Lysosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LIMPII/SR-B2 Antibody

Western Blot: LIMPII/SR-B2 Antibody [NB400-102]

Western Blot: LIMPII/SR-B2 Antibody [NB400-102]

Western Blot: SR-BII Antibody [NB400-102] - Western blot analysis of SR-BII in 1. Human heart lysate 2. Mouse heart lysate
Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-102]

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-102]

Immunocytochemistry/Immunofluorescence: SR-BII Antibody [NB400-102] - SR-BII antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).

Applications for LIMPII/SR-B2 Antibody

Application
Recommended Usage

Flow Cytometry

reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:25-1:100

Immunohistochemistry

1:25

Immunohistochemistry-Paraffin

1:25

Knockdown Validated

reported in scientific literature (PMID 31132962)

Western Blot

1:1000
Application Notes
In Western blot a band is observed at ~82 kDa in tissues that express SR-BII such as liver, testes, and adrenal glands. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LIMPII/SR-B2

Scavenger receptor class BI or SCARB1 gene belongs to CD36 receptor family and encodes for at least two mRNA splice variants which are SR-BI and SR-BII, among which SR-BII variant is the main isoform in terms of mRNA levels in certain tissues. For example, SR-BII mRNA is 48-fold higher than SR-BI in brain cells and protein levels reaching 10-15% of SR-BI in the liver. Similar differential SR-BII protein expression levels have also been detected in considerable amounts in other tissues, such as testes, retinal pigment epithelial cells, and spleen. SR-BII isoform differs from SR-BI only in its entirely different, yet highly conserved, cytoplasmic C-terminus which has been proposed to contains a signal responsible for marked alterations in cellular distribution as well as cellular trafficking of SR-BII compared with SR-BI. In contrast to SR-BI, SR-BII mediates the rapid uptake of significant amounts of HDL into endosomal recycling compartment by a pathway which is different from selective lipid uptake at the cell surface (mediated by SR-BI). Because SR-BII influence cellular cholesterol trafficking/homeostasis in a manner distinct from SR-BI, the relative expression as well as functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.

Long Name

Lysosomal Integral Membrane Protein II

Alternate Names

CD36L2, LPG85, SCARB2, SR-B2, SR-BII, SRB2

Entrez Gene IDs

950 (Human); 12492 (Mouse); 117106 (Rat)

Gene Symbol

SCARB2

UniProt

O35114

Additional LIMPII/SR-B2 Products

Product Documents for LIMPII/SR-B2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LIMPII/SR-B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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