LIMPII/SR-B2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB400-129
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Primate
Cited:
Human, Mouse, Rat, Canine
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence. [UniProt# O35114]
Localization
Lysosome membrane; Multi-pass membrane protein.
Marker
Lysosome Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for LIMPII/SR-B2 Antibody
Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129]
Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A) and aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated by dashed yellow line). Rhodamine phalloidin (red), which labels the cortical actin cytoskeleton, was used to show cell borders. Umbrella cells show an apparent, age-related increase in LIMP2 staining (white arrows). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0198817), licensed under a CC-BY license.Western Blot: LIMPII/SR-B2 Antibody [NB400-129]
Western Blot: LIMPII/SR-B2 Antibody [NB400-129] - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 (5), HEPG2 human liver lysate (6).Simple Western: LIMPII/SR-B2 Antibody [NB400-129]
Simple Western: LIMPII/SR-B2 Antibody [NB400-129] - CHAPS-solublized SVG-A (human astrocyte cells) and B103 (rat neuroblastoma cells) cell lyates, at the indicated concentrations, were probed with a 1:25 dilution of antibody. Image from verified customer review.Applications for LIMPII/SR-B2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:250
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
reported in scientific literature (PMID 23863627)
Immunoprecipitation
1:100
Western Blot
1:1000
Application Notes
In Western Blot, a band is observed at ~85 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 review rated 4 using NB400-129 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: LIMPII/SR-B2
Long Name
Lysosomal Integral Membrane Protein II
Alternate Names
CD36L2, LPG85, SCARB2, SR-B2, SR-BII, SRB2
Gene Symbol
SCARB2
Additional LIMPII/SR-B2 Products
Product Documents for LIMPII/SR-B2 Antibody
Product Specific Notices for LIMPII/SR-B2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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