LIN-28A Antibody (2S1I10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16843

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16843-100ul
NBP3-16843-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2S1I10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LIN-28A Antibodies »

Product Summary for LIN-28A Antibody (2S1I10)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LIN-28A (Q9H9Z2). MGSVSNQQFAGGCAKAAEEAPEEAPEDAARAADEPQLLHGAGICKWFNVRMGFGFLSMTARAGVALDPPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LIN-28A Antibody (2S1I10)

Western Blot: LIN-28A Antibody (2S1I10) [NBP3-16843]

Western Blot: LIN-28A Antibody (2S1I10) [NBP3-16843]

Western Blot: LIN-28A Antibody (2S1I10) [NBP3-16843] - Western blot analysis of extracts of various cell lines, using LIN-28A antibody (NBP3-16843) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]

Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]

Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843] - Immunofluorescence analysis of mouse testis using LIN-28A Rabbit mAb (NBP3-16843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]

Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843]

Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843] - Immunofluorescence analysis of rat testis using LIN-28A Rabbit mAb (NBP3-16843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Applications for LIN-28A Antibody (2S1I10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:2000 - 1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIN-28A

lin-28 homolog (C. elegans), also known as CSDD1, ZCCHC1. Entrez Protein NP_078950. LIN28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.

Long Name

RNA-binding Protein LIN-28

Alternate Names

CSDD1, LIN28, LIN28A, Tex17, ZCCHC1

Gene Symbol

LIN28A

Additional LIN-28A Products

Product Documents for LIN-28A Antibody (2S1I10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LIN-28A Antibody (2S1I10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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