Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all LIN-41 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TRIM71. The immunogen is located within amino acids 680 - 730 of TRIM71. Amino Acid Squence: CGEKGTKNGQFNYPWD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LIN-41/TRIM71 Antibody - BSA Free

Western Blot: LIN-41/TRIM71 AntibodyBSA Free [NBP1-76281]

Western Blot: LIN-41/TRIM71 AntibodyBSA Free [NBP1-76281]

Western Blot: LIN-41/TRIM71 Antibody [NBP1-76281] - Human brain tissue lysate with TRIM71/lin-41 antibody at (A) 1 and (B) 2 ug/mL.
LIN-41/TRIM71 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LIN-41/TRIM71 Antibody - BSA Free [NBP1-76281] -

Immunocytochemistry/ Immunofluorescence: LIN-41/TRIM71 Antibody - BSA Free [NBP1-76281] - Immunofluorescence of LIN-41/TRIM71 in Human Brain cells with LIN-41/TRIM71 antibody at 20 u/mL.
LIN-41/TRIM71 Antibody - BSA Free

Immunohistochemistry: LIN-41/TRIM71 Antibody - BSA Free [NBP1-76281] -

Immunohistochemistry: LIN-41/TRIM71 Antibody - BSA Free [NBP1-76281] - Immunohistochemistry of LIN-41/TRIM71 in human brain tissue with LIN-41/TRIM71 antibody at 5 u/mL.

Applications for LIN-41/TRIM71 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIN-41

TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development , and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.

Long Name

RNA-binding Protein LIN-41

Alternate Names

LIN41, TRIM71

Gene Symbol

TRIM71

UniProt

NP_001034200 (Human)

Additional LIN-41 Products

Product Documents for LIN-41/TRIM71 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LIN-41/TRIM71 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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