lin-54 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14854
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody maps to a region between residue 1 and 50 of human Lin-54 homolog using the numbering given in entry NP_919258.2 (GeneID 132660).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for lin-54 Antibody
Immunoprecipitation: lin-54 Antibody [NBP2-14854]
Immunoprecipitation: lin-54 Antibody [NBP2-14854] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-LIN54 antibody used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. LIN54 was also immunoprecipitated by rabbit anti-LIN54 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).Applications for lin-54 Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: lin-54
Additional lin-54 Products
Product Documents for lin-54 Antibody
Product Specific Notices for lin-54 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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