Lipase A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19360
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rhesus Macaque (98%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Lipase A. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (82%).
Localization
Lysosome
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Lipase A Antibody
![Immunocytochemistry/ Immunofluorescence: Lipase A Antibody [NBP2-19360]](https://resources.bio-techne.com/images/products/Lipase-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19360-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Lipase A Antibody [NBP2-19360]")
Immunocytochemistry/ Immunofluorescence: Lipase A Antibody [NBP2-19360]
Immunocytochemistry/Immunofluorescence: Lipase A Antibody [NBP2-19360] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: LAL stained by LAL antibody [N3C3] diluted at 1:500. Blue: Fluoroshield with DAPI.![Western Blot: Lipase A Antibody [NBP2-19360]](https://resources.bio-techne.com/images/products/Lipase-A-Antibody-Western-Blot-NBP2-19360-img0002.jpg "Western Blot: Lipase A Antibody [NBP2-19360]")
Western Blot: Lipase A Antibody [NBP2-19360]
Western Blot: Lipase A Antibody [NBP2-19360] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.![Western Blot: Lipase A Antibody [NBP2-19360]](https://resources.bio-techne.com/images/products/Lipase-A-Antibody-Western-Blot-NBP2-19360-img0004.jpg "Western Blot: Lipase A Antibody [NBP2-19360]")
Western Blot: Lipase A Antibody [NBP2-19360]
Western Blot: Lipase A Antibody [NBP2-19360] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAL antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.wApplications for Lipase A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: LIPA
Additional LIPA Products
Product Specific Notices for Lipase A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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