Lipin 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-53802

Novus Biologicals, part of Bio-Techne
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NB100-53802
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Lipin 3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KPKQKEDAVATD corresponding to internal region according to NP_075047.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Lipin 3 Antibody

Immunohistochemistry-Paraffin: Lipin 3 Antibody [NB100-53802]

Immunohistochemistry-Paraffin: Lipin 3 Antibody [NB100-53802]

Immunohistochemistry-Paraffin: Lipin 3 Antibody [NB100-53802] - (2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Lipin 3 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 3 ug/ml

Immunohistochemistry-Paraffin

2 - 3 ug/ml

Peptide ELISA

Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave bands at approx. 80 kDa band and 60 kDa band in human liver lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.6 kDa band according to NP_075047.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). IHC-P: Human thyroid gland shows staining of cytoplasm and cell membrane of epithelial cells surrounding the follicles.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lipin 3

Humans lipodystrophy is characterized by loss of body fat, fatty liver, hypertriglyceridemia, and insulin resistance. Mice carrying mutations in the fatty liver dystrophy (fld) gene have similar phenotypes. Through positional cloning, the mouse gene responsible for fatty liver dystrophy was isolated and designated Lpin1. The nuclear protein encoded by Lpin1 was named lipin. Lpin1 mRNA was expressed at high levels in adipose tissue and was induced during differentiation of preadipocytes. These results indicated that lipin is required for normal adipose tissue development and provided a candidate gene for human lipodystrophy. Through database searches, mouse and human EST and genomic sequences with similarities to Lpin1 were identified. These included two related mouse genes (Lpin2 and Lpin3) and three human homologs (LPIN1, LPIN2, and LPIN3). Human LPIN1 gene has been mapped to 2p25.; linkages of fat mass and serum leptin levels to this same region have been noted. Human LPIN2 and LPIN3 mapped to chromosomes 18p11 and 20q11-q12, respectively. The mouse genes encoding Lpin1, Lpin2, and Lpin3 mapped to chromosome 12, 17, and 2, respectively.

Long Name

Phosphatidate Phosphatase LPIN3

Alternate Names

LIPN3L, LPIN3, SMP2

Entrez Gene IDs

64900 (Human)

Gene Symbol

LPIN3

UniProt

NP_075047.1 (Human)

Additional Lipin 3 Products

Product Documents for Lipin 3 Antibody

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Product Specific Notices for Lipin 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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