Lipolysis Stimulated Lipoprotein Receptor Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89631
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Porcine, Rhesus Macaque
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot
Cited:
Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPRGWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGRSRAYMPPRSRSRDDLYDQDDS
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25753034). Primate (Rhesus macaques) reported in scientific literature (PMID: 26067369). Porcine reactivity reported in scientific literature (PMID: 24048008)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Lipolysis Stimulated Lipoprotein Receptor Antibody
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining in human stomach and kidney tissues . Corresponding LSR RNA-seq data are presented for the same tissues.Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]
Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Analysis in human cell lines A-431 and U-251MG using anti-LSR antibody. Corresponding LSR RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.Immunocytochemistry/ Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]
Immunocytochemistry/Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.Applications for Lipolysis Stimulated Lipoprotein Receptor Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Knockdown Validated
Reported in scientific publication (PMID: 32423802).
Simple Western
1:20
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue, internal validation.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue, internal validation.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LSR
Long Name
Lipolysis Stimulated Lipoprotein Receptor
Alternate Names
ILDR3, LISCH, LISCH7
Gene Symbol
LSR
Additional LSR Products
Product Documents for Lipolysis Stimulated Lipoprotein Receptor Antibody
Product Specific Notices for Lipolysis Stimulated Lipoprotein Receptor Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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