LIS1 Antibody (5A5)
Novus Biologicals, part of Bio-Techne | Catalog # H00005048-M03
Key Product Details
Species Reactivity
Human, Mouse, Porcine, Bovine, Sheep
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5A5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PAFAH1B1 (NP_000421, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDVNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSP
Specificity
PAFAH1B1 - platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa (5A5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for LIS1 Antibody (5A5)
![Western Blot: LIS1 Antibody (5A5) [H00005048-M03]](https://resources.bio-techne.com/images/products/LIS1-Antibody-5A5-Western-Blot-H00005048-M03-img0004.jpg "Western Blot: LIS1 Antibody (5A5) [H00005048-M03]")
Western Blot: LIS1 Antibody (5A5) [H00005048-M03]
Western Blot: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of PAFAH1B1 expression in transfected 293T cell line by PAFAH1B1 monoclonal antibody (M03), clone 5A5. Lane 1: PAFAH1B1 transfected lysatE (46.6 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: LIS1 Antibody (5A5) [H00005048-M03]](https://resources.bio-techne.com/images/products/LIS1-Antibody-5A5-Immunocytochemistry-Immunofluorescence-H00005048-M03-img0002.jpg "Immunocytochemistry/ Immunofluorescence: LIS1 Antibody (5A5) [H00005048-M03]")
Immunocytochemistry/ Immunofluorescence: LIS1 Antibody (5A5) [H00005048-M03]
Immunocytochemistry/Immunofluorescence: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of monoclonal antibody to PAFAH1B1 on HeLa cell. Antibody concentration 15 ug/ml
Sandwich ELISA: LIS1 Antibody (5A5) [H00005048-M03] - Detection limit for recombinant GST tagged PAFAH1B1 is 0.03 ng/ml as a capture antibody.
Applications for LIS1 Antibody (5A5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: LIS1
Alternate Names
LIS-1, LIS1PAF-AH alpha, LIS2, lissencephaly 1 protein, Lissencephaly-1 protein, MDCR, MDS, Miller-Dieker syndrome chromosome region, PAF acetylhydrolase 45 kDa subunit, PAFAH, PAF-AH 45 kDa subunit, PAFAH alpha, PAFAHA, platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa), platelet-activating factor acetylhydrolase IB subunit alpha, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD), platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa, platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)
Entrez Gene IDs
5048 (Human)
Gene Symbol
PAFAH1B1
UniProt
Additional LIS1 Products
Product Documents for LIS1 Antibody (5A5)
Product Specific Notices for LIS1 Antibody (5A5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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