Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 88C570

Format

Azide Free

Concentration

0.5 mg/ml

View all Livin Antibodies »

Product Specifications

Immunogen

Recombinant human livin protein was used as immunogen.

Specificity

The Livin antibody, clone 88C570 has been shown to recognize both Livin alpha (~39 kDa) and Livin beta (~37 kDa) isoforms (Nachmias et al, 2003). 2. The antibody also recognizes the 30 kDa (p30-Livin alpha) and 28 kDa (p28-Livin beta) apoptotic cleavage fragments of Livin alpha and beta, respectively (Nachmias et al, 2003). Apoptotic cleavage fragments of Livin have also been referred to as truncated livin (tLivin) (Nachmias et al, 2007). 3. The specificity of the antibody has been validated using Livin shRNA (Quiping et al, 2004).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Livin Antibody (88C570) - Azide Free

Western Blot: Livin Antibody (88C570)Azide Free [NBP2-33225]

Western Blot: Livin Antibody (88C570)Azide Free [NBP2-33225]

Western Blot: Livin Antibody (88C570) - Azide Free [NBP2-33225] - detection of Livin in A) Livin transfected cell lysate and B) Molt4 cell lysate. A protein band of apprximate molecular weight of 31 kDa is detected using this antibody. Image from the standard format of this antibody.

Applications for Livin Antibody (88C570) - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (Nachmias et al (2007))

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS - BSA free

Format

Azide Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Livin

Livin (BIRC7/KIAP) is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain (reviewed in Ma et al 2006 and Crnkovic-Mertens et al 2006). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis (reviewed in Wright et al. 2005). IAP proteins inhibit apoptosis by binding to and inhibiting caspases through their BIR domain(s). Some IAP members, like Liven, also have a RING-type finger motif at their carboxyl-terminal which may enhance anti-apoptotic activity. Many RING finger-containing IAPs possess E3 ubiquitin ligase activity, and it has been shown that Liven acts an E3 ubiquitin ligase for targeting the degradation of Smac/DIABLO. Smac/DIABLO functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Thus degradation of Smac/DIABLO is thought to be a mechanism to enhance Livin anti-apoptotic activity, thereby promoting cell survival. Two splicing variants of Livin, alpha and beta, have been identified. The two isoforms are thought to have different anti-apoptotic properties; however, there are conflicting reports are to whether they actually differ in their biological activities. There is accumulating evidence that Liven plays a significant role in a spectrum of tumor types and it is thought that Liven may have potential as a diagnostic and prognostic tumor marker. Liven is expressed at low levels in adult tissues, and relatively higher levels in developmental tissues and in many cancer cells. Certain cancer patients develop autoantibodies against liven and in a number of cancers, Liven is detected only in the tumors but not, or to substantially lower levels, in the corresponding normal adjacent tissue. This antibody recognizes Livin, both isoform alpha and isoform beta. Human Livin isoform alpha is a 298 amino acid (aa) protein, GenBank no. NP_647478. Human Livin isoform beta is a 280 aa protien, GenBank no. NP_071444.1. The antibody will also recognize other isoforms of Livin that contain the peptide immunogen sequence.

Alternate Names

BIRC7, KIAP, ML-IAP

Gene Symbol

BIRC7

Additional Livin Products

Product Documents for Livin Antibody (88C570) - Azide Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Livin Antibody (88C570) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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