Livin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56145

Novus Biologicals, part of Bio-Techne
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NB100-56145
NB100-56145SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Livin Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 180-230 of human Livin, isoform alpha and isoform beta was used as immunogen (NP_647478).

Specificity

Livin is a short-lived protein that is thought to be degraded by the ubiquitin-proteasome pathway. Thus users may want to do a time course when examining the effects of treatments on Livin expression. Users are encouraged to refer to the literature for additional information; for example, Ma et al. 2006. 2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Livin Antibody

Western Blot: Livin Antibody [NB100-56145]

Western Blot: Livin Antibody [NB100-56145]

Western Blot: Livin Antibody [NB100-56145] - Western blot analysis of livin isoforms expression in three of four paired samples of tumors (2 ACA and 1 ACC) and adjacent NAG that are showed also at mRNA levels. Whole cell lysate SK-MEL 28 was used as positive control and B-actin was used as internal standard. (D) Quantitative analysis of WB bands of all 15 paired samples of tumors and corresponding NAG (p < 0.0001 per trend). Each bar in the histograms represents the mean of the ratio livin A or livin B signal to B-actin signal. Statistical analysis by Kruskall-Wallis test and Mann-Whitney U test. N.s. = p not significant. Image collected and cropped by Citeab from the following publication (Livin/BIRC7 expression as malignancy marker in adrenocortical tumors. Oncotarget (2017) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Livin Antibody [NB100-56145]

Immunohistochemistry-Paraffin: Livin Antibody [NB100-56145]

Immunohistochemistry-Paraffin: Livin Antibody [NB100-56145] - Livin staining in normal adrenal glandLivin staining in normal adrenal gland (A). It is possible to recognize the three different zone of the adrenal gland: the zona glomerulosa (ZG), the zona fasciculata (ZF) and the zona reticularis (ZR). The Figures (B) and (C) are 20A- enlarged and detailed images of the 10A- ZG, ZF and ZR, respectively. Scale bar: 100 i1/4m. Image collected and cropped by Citeab from the following publication (Livin/BIRC7 expression as malignancy marker in adrenocortical tumors. Oncotarget (2017) licensed under a CC-BY license.
Western Blot: Livin Antibody [NB100-56145]

Western Blot: Livin Antibody [NB100-56145]

Western Blot: Livin Antibody [NB100-56145] - Analysis of full-length, recombinant human Livin using this antibody.
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Applications for Livin Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
Livin has been shown to undergo proteolytic cleavage during apoptosis. Thus the molecular weight of Livin observed on Western blots may vary depending on the presence of absence of cleavage fragments.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Neat whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Livin

Livin (BIRC7/KIAP) is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain (reviewed in Ma et al 2006 and Crnkovic-Mertens et al 2006). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis (reviewed in Wright et al. 2005). IAP proteins inhibit apoptosis by binding to and inhibiting caspases through their BIR domain(s). Some IAP members, like Liven, also have a RING-type finger motif at their carboxyl-terminal which may enhance anti-apoptotic activity. Many RING finger-containing IAPs possess E3 ubiquitin ligase activity, and it has been shown that Liven acts an E3 ubiquitin ligase for targeting the degradation of Smac/DIABLO. Smac/DIABLO functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Thus, degradation of Smac/DIABLO is thought to be a mechanism to enhance Livin anti-apoptotic activity, thereby promoting cell survival. Two splicing variants of Livin, alpha and beta, have been identified. The two isoforms are thought to have different anti-apoptotic properties; however, there are conflicting reports are to whether they actually differ in their biological activities. There is accumulating evidence that Liven plays a significant role in a spectrum of tumor types and it is thought that Liven may have potential as a diagnostic and prognostic tumor marker. Liven is expressed at low levels in adult tissues, and relatively higher levels in developmental tissues and in many cancer cells. Certain cancer patients develop autoantibodies against liven and in a number of cancers, Livin is detected only in the tumors but not, or to substantially lower levels, in the corresponding normal adjacent tissue. This antibody recognizes Livin, both isoform alpha and isoform beta. Human Livin isoform alpha is a 298 amino acid (aa) protein, GenBank no. NP_647478. Human Livin isoform beta is a 280 aa protein, GenBank no. NP_071444.1. The antibody will also recognize other isoforms of Livin that contain the peptide immunogen sequence.

Alternate Names

BIRC7, KIAP, ML-IAP

Entrez Gene IDs

79444 (Human); 329581 (Mouse); 296468 (Rat)

Gene Symbol

BIRC7

UniProt

Q96CA5

Additional Livin Products

Product Documents for Livin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Livin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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