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LLGL2 Antibody (4G2)

Novus Biologicals, part of Bio-Techne | Catalog # H00003993-M06

Novus Biologicals, part of Bio-Techne
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H00003993-M06

Key Product Details

Species Reactivity

Validated:

Human, Canine, Drosophila

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

LLGL2 (NP_004515, 101 a.a. ~ 199 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SLKVKGGASELQEDESFTLRGPPGAAPSATQITVVLPHSSCELLYLGTESGNVFVVQLPAFRALEDRTISSDAVLQRLPEEARHRRVFEMVEALQEHPR

Specificity

Human LLGL2 (4G2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for LLGL2 Antibody (4G2)

Western Blot: LLGL2 Antibody (4G2) [H00003993-M06]

Western Blot: LLGL2 Antibody (4G2) [H00003993-M06]

Western Blot: LLGL2 Antibody (4G2) [H00003993-M06] - Analysis of LLGL2 expression in transfected 293T cell line by LLGL2 monoclonal antibody (M06), clone 4G2. Lane 1: LLGL2 transfected lysatE (113 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: LLGL2 Antibody (4G2) [H00003993-M06]

Immunohistochemistry-Paraffin: LLGL2 Antibody (4G2) [H00003993-M06]

Immunohistochemistry-Paraffin: LLGL2 Antibody (4G2) [H00003993-M06] - Analysis of monoclonal antibody to LLGL2 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ug/ml
Sandwich ELISA: LLGL2 Antibody (4G2) [H00003993-M06] - Detection limit for recombinant GST tagged LLGL2 is approximately 0.03ng/ml as a capture antibody.

Applications for LLGL2 Antibody (4G2)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1ug/ml

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LLGL2

The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

HGLlethal giant larvae (Drosophila) homolog 2, human giant larvae homolog, lethal giant larvae homolog 2 (Drosophila), lethal(2) giant larvae protein homolog 2, LGL2

Entrez Gene IDs

3993 (Human); 217325 (Mouse)

Gene Symbol

LLGL2

Additional LLGL2 Products

Product Documents for LLGL2 Antibody (4G2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LLGL2 Antibody (4G2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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