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LLGL2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05135

Novus Biologicals, part of Bio-Techne
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NBP3-05135-100ul
NBP3-05135-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 620-819 of mouse LLGL2 (NP_663413.2). RQVFVKCTLHPSDQLALEGPLSRVKSLKKSLRQSFRRMRRSRVSSHKRRPGGPTGEAQAQAVNIKAERTGLQNMELAPVQRKIEARSAEDSFTGFVRTLYFADTYLRDSSRHCPSLWAGTNGGTVYAFSLRVPPAERRTDEPVRAEQAKEIQLMHRAPVVGILVLDGHNVPLPEPLEVAHDLSKSPDMQGSHQLLVVSEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LLGL2 Antibody - BSA Free

Western Blot: LLGL2 AntibodyBSA Free [NBP3-05135]

Western Blot: LLGL2 AntibodyBSA Free [NBP3-05135]

Western Blot: LLGL2 Antibody [NBP3-05135] - Analysis of extracts of various cell lines, using LLGL2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.
Immunocytochemistry/ Immunofluorescence: LLGL2 Antibody - BSA Free [NBP3-05135]

Immunocytochemistry/ Immunofluorescence: LLGL2 Antibody - BSA Free [NBP3-05135]

Immunocytochemistry/Immunofluorescence: LLGL2 Antibody [NBP3-05135] - Analysis of U2OS cells using LLGL2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for LLGL2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LLGL2

The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

HGLlethal giant larvae (Drosophila) homolog 2, human giant larvae homolog, lethal giant larvae homolog 2 (Drosophila), lethal(2) giant larvae protein homolog 2, LGL2

Gene Symbol

LLGL2

Additional LLGL2 Products

Product Documents for LLGL2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LLGL2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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