LMAN2 Antibody (02)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05830
![Western Blot: LMAN2 Antibody (02) [NBP3-05830]](https://resources.bio-techne.com/images/products/LMAN2-Antibody-02-Western-Blot-NBP3-05830-img0001.jpg "Western Blot: LMAN2 Antibody (02) [NBP3-05830]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 02
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LMAN2 (Uniprot#: Q12907; Asp 45-Arg 322).
Reactivity Notes
No cross-reactivity in ELISA with:
E.coli cell lysate
E.coli cell lysate
Specificity
No cross-reactivity in ELISA with: E.coli cell lysate
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for LMAN2 Antibody (02)
Western Blot: LMAN2 Antibody (02) [NBP3-05830]
Western Blot: LMAN2 Antibody (02) [NBP3-05830] - Lane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:40 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.)![Immunoprecipitation: LMAN2 Antibody (02) [NBP3-05830]](https://resources.bio-techne.com/images/products/LMAN2-Antibody-02-Immunoprecipitation-NBP3-05830-img0002.jpg "Immunoprecipitation: LMAN2 Antibody (02) [NBP3-05830]")
Immunoprecipitation: LMAN2 Antibody (02) [NBP3-05830]
Immunoprecipitation: LMAN2 Antibody (02) [NBP3-05830] - Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-LMAN2-His mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LMAN2-His mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDaApplications for LMAN2 Antibody (02)
Application
Recommended Usage
ELISA
1:1000-1:2000
Immunoprecipitation
4-6 uL/mg of lysate
Western Blot
1:500-1:2000
Application Notes
This antibody will detect Human LMAN2 in ELISA pair set (Catalog: # NBP2-79398). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP3-05831).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LMAN2
Alternate Names
C5orf8, chromosome 5 open reading frame 8, Glycoprotein GP36b, GP36B, Lectin mannose-binding 2, lectin, mannose-binding 2, vesicular integral protein of 36 kDa, vesicular integral-membrane protein VIP36, VIP36
Gene Symbol
LMAN2
Additional LMAN2 Products
Product Documents for LMAN2 Antibody (02)
Product Specific Notices for LMAN2 Antibody (02)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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